https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21729 Fzr1 knockout mouse embryos show normal preimplantation development but die due to a lack of endoreduplication needed for placentation. However, interpretation of the role of FZR1 during this period is hindered by the presence of maternal stores. In this study, therefore, we used an oocyte-specific knockout to examine FZR1 function in early mouse embryo development. Maternal FZR1 was not crucial for completion of meiosis, and furthermore viable pups were born to Fzr1 knockout females mated with normal males. However, in early embryos the absence of both maternal and paternal FZR1 led to a dramatic loss in genome integrity, such that the majority of embryos arrested having undergone only a single mitotic division and contained many γ-H2AX foci, consistent with fragmented DNA. A prominent feature of such embryos was the establishment of two independent spindles following pronuclear fusion and thus a failure of the chromosomes to mix (syngamy). These generated binucleate 2-cell embryos. In the 10% of embryos that progressed to the 4-cell stage, division was so slow that compaction occurred prematurely. No embryo development to the blastocyst stage was ever observed. We conclude that Fzr1 is a surprisingly essential gene involved in the establishment of a single spindle from the two pronuclei in 1-cell embryos as well as being involved in the maintenance of genomic integrity during the mitotic divisions of early mammalian embryos.]]> Wed 11 Apr 2018 11:35:19 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:41671 k_eff due to the expansion of the clay aggregates with increasing the as-compacted degree of saturation. The increase in the as-compacted degree of saturation in loose samples (dry density ≤1·3 Mg/m³) produces an enhancement in the proportion of macro pores, thus increasing k_eff, as a consequence of modifications in the pore size distribution. A threshold value has been identified, above which further increase in degree of saturation causes a reduction in the proportion of macro pores, and therefore in k_eff. A new proposal for estimating the air permeability is proposed in this paper based on the determination of a pore size parameter obtained from MIP data. The proposed approach seems capable of describing the evolution of air permeability for the whole spectrum of compaction states, including specimens subjected to wetting and drying paths.]]> Wed 10 Aug 2022 13:37:24 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:50915 Wed 06 Mar 2024 14:20:42 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33570 Wed 05 Aug 2020 11:21:21 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:45625 Wed 02 Nov 2022 14:56:15 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54515 Tue 27 Feb 2024 15:46:17 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38362 Tue 22 Feb 2022 14:09:32 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:54229 Tue 13 Feb 2024 12:17:52 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28381 Sat 24 Mar 2018 07:36:03 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31108 3 and a porosity range of 0.79–0.93 for expanded perlite particle sizes between 3 and 4 mm, a compaction ratio range of 1–3.56, and a pure sodium silicate content range of 0.05–0.35 g/ml in dilution of water for binder.]]> Sat 24 Mar 2018 07:35:06 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:28321 Sat 24 Mar 2018 07:25:14 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:30421 Mon 23 Sep 2019 13:58:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20932 Fri 21 Jun 2019 18:23:21 AEST ]]>